What's the difference between qubit and nanodrop?

Posted September 26, 2022

Cell Structures and Organelles

DNA and RNA Quantitation

Fluorescence Measurements and Instruments

Answer

The differences between qubit and nanodrop are listed in the table below.

Basis of differentiation	Qubit fluorometer	Nanodrop spectrophotometer
Sample volume requirement	1-10 microliters	0.5- 1 microliter
Principle	Fluorochrome chemistry	Spectroscopic analysis
Sample preparation	Required	Not required
Sample processing time	2-5 minutes	< 1 minute
Quanlification range	10 pg to 200 ng	10 nanograms to 10 micrograms

Additional resources

Comparison of DNA Quantification Methods for Next Generation Sequencing

Portelite™ Fluorimetric High Sensitivity DNA Quantitation Kit *Optimized for CytoCite™ and Qubit™ Fluorometers*

Spexyte™ Micro pH Probe *Optimized for Nanodrop*

Fluorescence Measurements and Instruments

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