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AAT Bioquest

What is the structure of green fluorescent protein (GFP)?

Posted July 9, 2024


Answer

Green Fluorescent Protein possesses a cylindrical shape formed by 11 strands of beta-sheet arranged around an alpha-helix situated inside. Additionally, there are short helical segments located at both ends of the cylinder. This unique structure makes up a novel protein fold known as the beta-can. Two individual units of the protein come together tightly to form a dimer when observed in its crystal form. 

Additional resources

Green fluorescent protein: A perspective

Fluorescent Proteins

Purified Rabbit Anti-GFP Antibody *Polyclonal*