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AAT Bioquest

What is the protein concentration of proteinase K (molecular biology grade)?

Posted September 7, 2023


Answer

The protein concentration of proteinase K is 20 mg/ml, as determined by UV absorption at 280 nm. Its unit activity is 800 U/ml. Protein concentration is determined by dividing the digest solution absorbance by the calculated epsilon (M) (280). The absorptivity of a native protein is then calculated by dividing the absorbance of the intact protein solution by the concentration value calculated for the digest solution.

Additional resources

Spectroscopic determination of protein concentrations from proteinase K digests

Protein Concentration Calculator

Enzymes

Amplite® Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*