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AAT Bioquest

What is the process of making ATP via chemiosmotic coupling?

Posted June 6, 2024


Answer

Oxidative phosphorylation is the method by which mitochondria produce ATP, utilizing chemiosmosis. In chemiosmosis, the energy released from the electron transport chain reactions is utilized to transport hydrogen ions across the inner mitochondrial membrane, creating an electrochemical gradient. This gradient drives the movement of hydrogen ions back into the mitochondrial matrix through a protein channel known as ATP synthase. As hydrogen ions pass through ATP synthase, ADP is converted into ATP. During aerobic glucose catabolism, chemiosmosis is responsible for producing approximately 90% of the ATP generated.

Additional resources

ATP and reaction coupling

ATP & ADP

PhosphoWorks™ Fluorimetric ATP Assay Kit