What is the procedure of the pour plate method?
Posted January 9, 2024
Answer
The procedure of the pour plate method is described in the steps below.
- Sample Preparation: If it's solid or semi-solid, dissolve it in sterile water or broth to form a liquid solution. For liquid samples, create serial dilutions to achieve a microbial concentration between 20-300 CFU/mL.
- Inoculation: To inoculate, open the lids of the petri dishes and pour 1 mL of the diluted sample. Heat the agar slightly and pour approximately 15-18 mL onto the sample. Ensure the agar is at an ideal temperature. Close the dish lid and gently swirl to mix.
- Alternatively, mix the diluted sample into the agar medium and pour this mixture into the petri dish, although this method is less common.
- Allow the plate to solidify after the inoculation process (approximately 10 minutes)
- Invert the plate and place it in an incubator set at the ideal temperature (37°celsius) for 24-48 hours.
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