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AAT Bioquest

What is specificity in chromatography?

Posted May 8, 2024


Answer

Specificity in chromatography refers to the ability of the method to separate and detect individual components of a mixture accurately and selectively.  It is demonstrated by the clear separation of analyte peaks from other peaks in the chromatogram, indicating the ability of the method to distinguish between different components. Specifically, it can be assessed by the resolution of two nearest-eluting samples.

Additional resources

Validation of the Specificity of Chromatographic Methods: Key Points and Practical Recommendations

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