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What is Propidium Monoazide (PMA)?

Posted July 17, 2024


Chemical Reagents
Cell Viability Assays
Cellular Structures and Organelles
DNA and RNA Quantitation
Physiological Probes
Plasma Membrane
Polymerase Chain Reaction (PCR)
Answer

PMA or Propidium Monoazide is a highly photoreactive, membrane-impermeable, DNA-intercalating dye that belongs to the phenanthridine family. It is widely used in viability PCR (vPCR) assays to discriminate between viable and nonviable microbial cells. 

Additional resources

Applicability of propidium monoazide (PMA) for discrimination between living and dead phytoplankton cells

Polymerase Chain Reaction (PCR)

Cell Viability Assays

Calcein, AM *UltraPure grade* *CAS 148504-34-1*

Related questions
How does viability PCR (vPCR) work?
What are the commonly used viability PCR reagents?
What is viability PCR (vPCR)?
What are the applications of viability PCR (vPCr)?
What are the factors that might cause the overestimation of live cells in PMA-treated samples?
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