What is chemiluminescence immunoassay?

Chemiluminescence immunoassay (CLIA) is a type of immunoassay technique that is based on the conversion of chemical energy into the emission of visible light resulting from a hydrolysis or oxidation reaction. In this process, antibodies or antigens are directly labeled with chemiluminescent agents. The emission of light resulting from the chemical reaction between the enzyme-labeled antibodies or antigens allows researchers to detect and quantify specific substances such as proteins or hormones in biological samples.