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AAT Bioquest

What is cell dissociation?

Posted December 15, 2023


Answer

Cell dissociation refers to the method used during cell passaging to separate cells from the treated surface, resulting in the formation of suspensions. These suspensions are crucial for purposes such as reseeding for subcultures, cell propagation, and cell counting. Cell dissociation methods typically involve using specific enzymes to disrupt the attachment between cells and the substrate, facilitating their release into suspensions. 

Additional resources

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Solubility Reference Table

Serial Dilution Calculator and Planner

HHBS [Hanks' Buffer with 20 mM Hepes]