What happens if there are too many adaptors attached to a DNA fragment? | AAT Bioquest

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What happens if there are too many adaptors attached to a DNA fragment?

Posted April 18, 2024

DNA and RNA Quantitation DNA Fragmentation Gene Expression Analysis & Genotyping Nucleic Acids Nucleus Sanger Sequencing

Answer

Excess adapters can lead to unwanted dimer formations, which may interfere with subsequent sequencing steps. If left unremoved, these contaminants and incorrectly sized fragments can reduce the efficiency and quality of sequencing.

Additional resources

Adapter dimers causes, effects, and how to remove them DNA Fragmentation Gelite™ Green Nucleic Acid Gel Staining Kit

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