What downstream applications can I use purified mRNA for?

One downstream application is expression analysis. Purified RNA is used in expression analysis to study gene expression levels and understand gene activity under various conditions. Techniques such as microarrays, quantitative PCR (qPCR), and RNA sequencing (RNA-Seq) measure the abundance of specific mRNA transcripts. This helps researchers identify genes that are upregulated or downregulated in response to different stimuli. Another downstream application is microinjections. Purified RNA can be injected directly into cells or embryos through microinjections to study RNA function; this precise delivery method uses fine needles or microinjectors. Another downstream application is transient transfection. Purified RNA is utilized in transient transfections to temporarily introduce RNA (such as mRNA) into cells for studying gene function. Methods like lipofection, electroporation, or viral vectors are used for delivery. Purified RNA is also used in various in vitro assays to study RNA characteristics. This includes RNA structure studies to understand RNA folding functional roles and in vitro translation to produce proteins from RNA in cell-free systems.