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AAT Bioquest

What does the isoelectric point tell me?

Posted May 24, 2024


Answer

When the pH of the solution surpasses the isoelectric point, the proteins are predominantly negatively charged resulting in repulsion between similarly-charged molecules.The isoelectric point (pI) denotes the pH at which a molecule holds zero electric charge. This concept holds special significance for zwitterionic compounds such as proteins, amino acids, and peptides.

Additional resources

IPC 2.0: prediction of isoelectric point and pKa dissociation constants

Isoelectric Point Calculator

pKa and pKb Reference Table