What do the primers involved in bridging PCR do?

The bridging primer is designed to bind to a region shared by both template molecules, allowing amplification across the region of sequence similarity. These primers are designed to hybridize to the ends of the DNA fragments that need to be bridged or connected. They contain sequences that are complementary to the ends of the DNA fragments, allowing them to anneal and serve as a template for DNA synthesis. The BP is created by combining the 5'-end of the OWP with the 5'-part of the IWP. The bridging PCR process involves three rounds of amplification reactions. First, the IWP amplifies the target sequence. Next, the BP, along with the OWP, amplifies the non-target product, which is then end-lengthened by the BP. Finally, the OWP amplifies the modified non-target product. These steps are repeated sequentially with three nested sequence-specific primers (SSP).