What do I need for my western blot membrane blotting?

The materials needed to carry out a western blot experiment include: blotting paper, transfer buffer, transfer membrane, a power supply, a tank transfer system, and alcohols (e.g. methanol, isopropanol, ethanol). 

• Blotting paper is an absorbent material used to facilitate the transfer of proteins from the gel to the membrane during Western blotting. It helps maintain contact between the gel and the membrane. 
• Transfer buffer is a specialized buffer solution used to facilitate the transfer of proteins from the gel to the membrane during Western blotting. It typically contains ions to maintain pH and ionic strength. 
• Transfer membranes are semi-permeable membranes made of materials such as polyvinylidene difluoride (PVDF) or nitrocellulose. They are used to immobilize proteins transferred from the gel and provide a surface for subsequent antibody binding and detection. 
• Alcohols are commonly used in Western blotting protocols for various purposes such as hydrating the membrane and enhancing protein binding. 
• A tank transfer system is an instrument used for the electroblotting step of Western blotting. It consists of a chamber filled with transfer buffer, electrodes, and a platform for holding the gel and membrane assembly. 
• The power supply applies an electric current to drive proteins from the gel onto the membrane.