What concentration should I use protease inhibitors?

Protease inhibitor

Optimal concentration

Aprotinin

1 µg/ml (0.15 µM)

Leupeptin

0.5 µg/ml (1 µM)

Pepstatin

0.7 µg/ml (1 µM)

PMSF

85 µg/ml (0.5 mM)

Na2-EDTA

0.35 mg/ml (0.75 mM)

Pefabloc

0.5 mg/ml (2 mM)