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AAT Bioquest

What can I do with whole plasmid sequencing?

Posted October 2, 2024


Answer

Whole plasmid sequencing uses long-read sequencing technology, specifically nanopore technology, to read the entire DNA sequence of a plasmid. 

Whole plasmid sequencing can be used in a wide range of applications including:

  • Plasmid verification
  • Sequencing difficult regions such as GC-rich regions and repetitive sequences 
  • Resequencing whole genomes
  • Identifying structural changes or genetic material degradation that may occur during replication thereby enabling research into overcoming these structural instabilities and developing safe and effective molecules for disease treatments
  • Identifying of pathogens, including anti-microbial resistance genes rapidly, potentially within a few hours
  • Metagenomic analysis of long reads
Additional resources

Using a combination of short- and long-read sequencing to investigate the diversity in plasmid- and chromosomally encoded extended-spectrum beta-lactamases (ESBLs) in clinical Shigella and Salmonella isolates in Belgium

Sanger Sequencing

Next Generation Sequencing (NGS)