There are also various limitations of using serial dilution in microbiology. 

• Serial dilution doesn't separate bacteria similar to a streak plate, since it doesn't physically isolate or separate bacteria. 
• It also requires a longer period of time, and overnight incubation is sometimes required, limiting the efficiency of this technique. 
• Errors may occur in transferring the sample during propagation, causing less accurate and less precise dilution. This impacts the highest dilution the most, resulting in the least accuracy. 
• This technique requires highly skilled researchers and experts proficient in aseptic techniques.