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What are the limitations of post-electrophoresis staining?

Posted May 2, 2023


Agarose gel electrophoresis
DNA and RNA
Gel Electrophoresis
Nucleic Acids
Nucleus
Protein
polyacrylamide gel
Answer

Main limitations of post-electrophoresis staining include: 

  • Larger amounts of dye required
  • Adds time to the workflow
  • More hazardous waste generated when using dyes such as ethidium bromide
Additional resources

Analysis of drug interactions with serum proteins and related binding agents by affinity capillary electrophoresis: A review

Gel Electrophoresis

Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution*

Related questions
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How is a DNA ladder visualized in gel electrophoresis?
What are the advantages of in-gel staining?
What are the limitations of in-gel staining?