What are the general steps of sample preparation for NGS?

The general steps of sample preparation are described below.

1. The initial step in sample preparation is isolating nucleic acids through physical, enzymatic, or chemical methods; ideally, isolating nucleic acids from a homogenous population of cells from an in vitro culture is optimal.
2. Determine the NGS application, whether it's  Exome-Seq, WGS, RNA-Seq. Or Methyl-Seq.
3. Select the appropriate tissue for RNA or DNA extraction. Considerations for tissue collection include location,  preservation methods and timing. Once collected, tissues can be stored in a freezer at -80°C until extraction.
4. Select or develop an appropriate extraction protocol for DNA or RNA, considering kits, reagents, and understanding the underlying biology of each step.
5. Assess the quality of the extraction using techniques like spectrophotometry and fluorescence checking for concentration and purity
6. Prepare and ship the samples for sequencing: dissolve them in TE buffer or 10mM Tris pH 8.0 buffers and store them on blue ice at 4°C until shipment