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What are the disadvantages of using ssDNA probes for performing in situ hybridization?

Posted March 27, 2024


DNA and RNA Quantitation
FISH
Fluorescence
Nucleic Acids
Nucleus
Physiological Probes
in situ hybridization
Answer

The key disadvantages are that it is expensive. ssDNA probes can also be time consuming.

Additional resources

Technical Review: In Situ Hybridization

Fluorescent in situ hybridization (FISH)

ReadiLink™ iFluor® 488 FISH Fluorescence Imaging Kit

Related questions
How does Fluorescence in situ hybridization (FISH) work?
What are the advantages of Fluorescence in situ hybridization (FISH)?
What are the advantages of using dsDNA probes for performing in situ hybridization?
What are the advantages of using RNA probes for performing in situ hybridization?
What are the advantages of using ssDNA probes for performing in situ hybridization?