What are the disadvantages of spread plate technique?
Posted January 9, 2024
Answer
There are various limitations of the spread plate method.
- An additional instrument is required, such as a spreader, for even distribution.
- For solid or semisolid samples, they must be converted into suspensions before inoculation.
- It requires liquid or easily soluble samples, which often need serial dilution.
- It may not adequately support the growth of certain microorganisms like anaerobes and micropiles.
- Sterilization of tools is also required after each plate to avoid cross-contamination.
- This method also requires pre-solidified specific media beforehand, and thus prior knowledge is necessary about potential microorganisms in the sample.
Additional resources