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AAT Bioquest

What are the disadvantages of spread plate technique?

Posted January 9, 2024


Answer

There are various limitations of the spread plate method. 

  • An additional instrument is required, such as a spreader, for even distribution. 
  • For solid or semisolid samples, they must be converted into suspensions before inoculation. 
  • It requires liquid or easily soluble samples, which often need serial dilution
  • It may not adequately support the growth of certain microorganisms like anaerobes and micropiles. 
  • Sterilization of tools is also required after each plate to avoid cross-contamination. 
  • This method also requires pre-solidified specific media beforehand, and thus prior knowledge is necessary about potential microorganisms in the sample.     
Additional resources

Spread Plate Method- Definition, Principle, Procedure, Uses

Cell Sample Preparation

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