What are the differences between viable and nonviable cells?

Question

Accepted Answer

Basis of differentiation
Viable cells
Nonviable cells

Definition
Are living cells that occur naturally in regular cell culture
Are nonliving cells that occur naturally in regular cell culture

Ability to grow
Have the ability to grow
Cannot grow

Ability to divide and reproduce
Have the ability to divide and reproduce
Cannot divide or reproduce

Cell membrane feature
Have an intact cell membrane 
Have a permeable cell membrane

Staining
Methylene blue is the stain used to detect viable cells
Trypan blue, Erythrosin B, and Nigrosin are stains used to detect nonviable cells

Effect of flow cytometry technique
Viable cells emit light when passed through a cytometer
Nonviable cells do not emit any light when passed through a cytometer

Effect of freezing on cells
Cells frozen with cryoprotectants are viable
Snap-frozen cells are nonviable

Basis of differentiation	Viable cells	Nonviable cells
Definition	Are living cells that occur naturally in regular cell culture	Are nonliving cells that occur naturally in regular cell culture
Ability to grow	Have the ability to grow	Cannot grow
Ability to divide and reproduce	Have the ability to divide and reproduce	Cannot divide or reproduce
Cell membrane feature	Have an intact cell membrane	Have a permeable cell membrane
Staining	Methylene blue is the stain used to detect viable cells	Trypan blue, Erythrosin B, and Nigrosin are stains used to detect nonviable cells
Effect of flow cytometry technique	Viable cells emit light when passed through a cytometer	Nonviable cells do not emit any light when passed through a cytometer
Effect of freezing on cells	Cells frozen with cryoprotectants are viable	Snap-frozen cells are nonviable

What are the differences between viable and nonviable cells?

Posted September 17, 2022