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What are the differences between static quenching and dynamic quenching?
Posted January 13, 2023
Basis of differentiation |
Static quenching |
Dynamic quenching |
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Definition |
Static quenching occurs when molecules create a complex in the ground state, before excitation occurs |
Dynamic quenching is the interaction of an excited state fluorophore with the quencher that concludes in deactivation of the fluorophore to the ground state |
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Effect of temperature |
If temperature increases, the rate of static quenching will decrease (chemical stability changes) |
If temperature increases, the dynamic quenching rate will also increase |
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Lifetime |
The excited state lifetime is not variable |
The excited state lifetime is variable |
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Measurement technique |
It is measured using a spectrophotometer |
It is measured using a spectrofluorometer |
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Fluorophore absorption spectrum |
Fluorophore absorption spectrum is distorted |
Fluorophore absorption spectrum remains not distorted |