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AAT Bioquest

What are the differences between serial dilution and parallel dilution?

Posted January 9, 2024


Answer

Basis of differentiation

Serial dilution

Parallel dilution

Definition

Serial dilution involves a step-by-step process of repeatedly diluting a concentrated solution to achieve a more manageable concentration 

Is the simultaneous dilution of separate samples to achieve varying concentrations 

Dilution factor

Is the same dilution factor in each step

Is a variable dilution factor among different samples

Flexibility

Offers limited flexibility, as it is a sequential process

Is more flexible as multiple dilutions can be prepared simultaneously 

Time consumption

Is time-consuming, since it has numerous steps

Is less time-consuming, as all dilutions happen concurrently

Additional resources

Serial dilutions and standard curve

Serial Dilution Calculator and Planner