What are the differences between nitrocellulose membrane and nylon membrane?

Basis of differentiation

Nitrocellulose Membrane

Nylon membrane

Definition

Is a sticky membrane utilized for immobilizing proteins and nucleic acids in blotting

Is a hydrophilic, heat-resistant and mechanically strong membrane used to immobilize macromolecules

Composition

Is composed of nitrated cellulose

Is typically made of a synthetic polymer called nylon, specifically nylon-66 or nylon-6

Affinity

Has a strong affinity for proteins

Has a high affinity for nucleic acids

Pore size

Pore sizes can be either 0.2 µm or 0.45 µm

Pore sizes range from 0.20 to 1.2 µm

Durability

Are brittle and not reusable

Are mechanically stronger than nitrocellulose membranes

Use

Prewetting is required

Prewetting is not required

Mechanism

Electrophoretic transfer is primarily used for the transfer of proteins

Capillary transfer is primarily used in the transfer of proteins

Reprobing

Are harder to reprobe compared to nylon membranes

Are comparatively easier to reprobe