What are the differences between fluorescence and luminescence?
Posted January 12, 2023
Basis of differentiation |
Fluorescence |
Luminescence |
Definition |
Fluorescence is the absorption of energy by atoms or molecules followed by the emission of light or electromagnetic radiation |
Is a spontaneous emission of light by a substance not resulting from heat |
Types |
TIRF, wide-field epifluorescence microscopy, and confocal microscopy |
Photoluminesce, chemiluminescence, crystalloluminescence |
Lifetime |
The lifetime of the excited state is very short, and photons are emitted from the atoms immediately after incident photons are absorbed |
A photon may be illuminated at any given time, and its lifetime may very from process to process |
Applications |
Mineralogy, fluorescent labeling, use in dyes |
Emissive displays, fluorescence lamps, LEDs |
Photoluminescence, Phosphorescence, and Fluorescence Spectroscopy
Amplite® Luciferase Reporter Gene Assay Kit *Maximized Luminescence*
PhosphoWorks™ Luminometric ATP Assay Kit *Extended Luminescence*