What are the differences between AFLP and RFLP?

Question

Accepted Answer

Basis of differentiation

AFLP

RFLP

Definition

Is a PCR-based method used to create distinctive genomic fingerprints by selectively amplifying specific DNA fragments after digestion.

Is a genetic marker that relies on the generation of DNA fragments of varying lengths after enzymes s are treated with specific restriction enzymes

Cost

Costs more

Costs less

Reliability

Is more reliable

Is less reliable

Sequence knowledge

Sequence knowledge is not required beforehand

Prior sequence knowledge is required

Polymorphism detection efficiency

Has a higher efficiency in detecting polymorphisms

Has a lower efficiency in detecting polymorphisms

Basis of differentiation	AFLP	RFLP
Definition	Is a PCR-based method used to create distinctive genomic fingerprints by selectively amplifying specific DNA fragments after digestion.	Is a genetic marker that relies on the generation of DNA fragments of varying lengths after enzymes s are treated with specific restriction enzymes
Cost	Costs more	Costs less
Reliability	Is more reliable	Is less reliable
Sequence knowledge	Sequence knowledge is not required beforehand	Prior sequence knowledge is required
Polymorphism detection efficiency	Has a higher efficiency in detecting polymorphisms	Has a lower efficiency in detecting polymorphisms