What are the common transfer buffers for western blots ?

Posted September 13, 2024

Answer

Transfer buffers are solutions used in Western blotting to transfer the separated proteins from the gel to a solid support, which facilitates the detection and analysis of specific proteins. Three common transfer buffers for Western blotting include:

Towbin Buffer
Dunn Carbonate Buffer
CAPS Buffer

Additional resources

Transfer Buffer Containing Methanol Can Be Reused Multiple Times in Protein Electrotransfer

Buffers and Lab Consumables

Buffer Preparations and Recipes

Western Blotting Assays

iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*