What are the common bacteria enumeration techniques?
Posted September 18, 2023
Answer
Bacteria enumerating techniques are used to determine approximately how many bacterial cells are present in a sample. The four most common bacterial enumeration techniques include:
- Direct Cell Count: This process uses counting chambers to directly count the number of bacteria in a liquid media or colonies on a plate.
- Indirect Cell Count: In this process, the plate count method is used to estimate the concentration of bacterial cells in a sample indirectly by quantifying bacterial colonies.
- Viable Cell Count: This method focuses on counting only metabolically active bacterial cells that are actively growing in the culture medium.
- Total Cell Count: The total number of bacterial cells, including metabolically active as well as metabolically inactive microbes are counted in this method.
Additional resources
Schrödinger’s microbes: Tools for distinguishing the living from the dead in microbial ecosystems