What are the chemical properties of acridine orange?

Posted March 12, 2024

Cell Cycle Assays

Cellular Structures and Organelles

DNA and RNA Quantitation

Flow Cytometry Reagents

Answer

Acridine orange is a cell-permeable fluorescent dye with these chemical properties:

Molecular Formula = C17H19N3
Molecular Weight = 265.35 g/mol
Dark orange to brown powder
Soluble in water, ethanol, and dimethyl sulfoxide
Produces green fluorescence on binding with DNA and orange fluorescence on binding to RNA and green fluorescence when it binds to DNA

Additional resources

Acridine Orange Stain for Determining Intracellular Enteropathogens in HeLa Cells

Fluorescence Microscopy

Flow Cytometry Reagents

Acridine orange *10 mg/mL solution in water*

Related questions

What are the advantages of acridine orange stain?

What are the applications of acridine orange stain?

What are the limitations of acridine orange stain?

What is the general procedure of acridine orange staining?

What is the principle of acridine orange stain?