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AAT Bioquest

What are the benefits of ionic binding techniques?

Posted February 22, 2024


Answer

Benefits of ionic binding techniques 

  • It is a relatively simple enzyme immobilization that requires minimum input. 
  • Ionic bonding takes place under mild conditions such as room temperature and neutral pH, which helps preserve enzyme activity. 
  • The ionic bonding can be reversed easily by altering the pH or by salting out of the enzymes. 
  • It is easy to sustain an optimum pH though the entire reaction by controlling the acidity/alkalinity of the mixture, as the matrix that inactivates the enzyme is steadily charged. 
Additional resources

An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes

Enzymes

Amplite® Fluorimetric Coenzyme A Quantitation Kit *Green Fluorescence*