What are the advantages of using mass spectrometry (MS) to detect phosphorylated Proteins?

There are various advantages of using MS to detect phosphorylated proteins. MS also allows for the simultaneous identification and quantification of multiple phosphorylated proteins in a single experiment. Its data collection is typically completed in less than 1 day, which makes it time efficient. It is also antibody-independent, and exhibits high sensitivity as it offers exact information about the location of phosphorylation within a protein sequence. MS also has the ability to differentiate between protein isoforms, and provides a large dynamic range in its analysis. One can consider MS to also be a quantitative measurement because it determines the molecular composition and structure of analytes based on the m/z values.