What are the advantages of the LAMP method?
Posted June 21, 2022
LAMP (loop-mediated isothermal amplification is a DNA amplification technique that offers several advantages over PCR (polymerase chain reaction), which is a more traditional DNA amplification technology. Advantages of the LAMP method include:
● DNA amplification occurs with greater efficiency at isothermal conditions (constant temperature of 63 to 65°C)
● Enables specific amplification by designing 4 primers to recognize 6 distinct regions on the target gene
● No requirement for thermal denaturation of double stranded DNA
● Amplification and detection is far more rapid than conventional DNA amplification techniques such as PCR
● Is cheaper than other DNA amplification methods because there is no need for sophisticated equipment or special reagents
● Can be used to amplify RNA templates with the addition of reverse transcriptase
Loop-mediated isothermal amplification of DNA