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AAT Bioquest

What are the advantages of polymer-based methods?

Posted May 8, 2024


Answer

This polymer system simplifies the IHC staining process by condensing multiple steps into a single procedure, thus enhancing efficiency. Because polymer-based IHC methods do not involve biotin, there is no risk of interference with endogenous biotin. This advantage eliminates a common concern associated with ABC and LSAB staining methods. Additionally, The polymer chain offers numerous binding sites, which is another reason it has higher sensitivity and specificity in comparison to ABC and LSAB methods.

Additional resources

An Introduction to the Performance of Immunohistochemistry

Immunohistochemistry (IHC)

ReadiUse™ Stayright™ Purple *HRP Chromogen Premixed with Hydrogen Peroxide*