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AAT Bioquest

What are the advantages of fluorescence spectroscopy?

Posted July 25, 2024


Answer

Fluorescence spectroscopy offers several advantages including: 

  • Higher Sensitivity – It is about 1,000 times more sensitive than other spectroscopy techniques and can detect the smallest traces of a substance. 
  • Higher Specificity – It selectively analyzes only the compound of interest, which absorbs the excitation light and emits light at a specific wavelength. 
  • Provides quantitative information – The fluorescence signal is proportional to the concentration of the emitting substances in the sample. 
  • Provides qualitative information – Emission spectra provide an insight into the electronic ground state, while excitation spectra provide an insight into the electronic excited state. 
  • Higher versatility – Fluorescence instruments can be used to obtain several types of fluorescence measurements, as well as chemiluminescence, bioluminescence, and phosphorescence measurements.
  • Reduced background signal – The emitted light is read at a 90-degree angle to the exciting light, which reduces background light. 
  • Rapidity – Fluorescence experiments can be conducted quickly
  • Less destructive – After a fluorescence experiment, the sample remains largely intact. This is unlike some other spectroscopic techniques such as mass spectrometry. 
Additional resources

Single-Molecule Fluorescence Spectroscopy of Photosynthetic Systems

Spectrum Viewer