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AAT Bioquest

What are the advantages of dye exclusion assays?

Posted March 21, 2024


Answer

Dye exclusion assays offer distinct benefits for chemosensitivity testing due to simplicity and ability to detect cell kill in nondividing cells, requiring only small cell numbers. These assays are able to accurately and directly detect dead cells in non dividing populations. Additionally, these assays tend to be inexpensive. 

Additional resources

Cell Viability Assessment: Toward Content-Rich Platforms

Trypan Blue Dye Exclusion Assay

Cell Viability Assays

Trypan Blue, sodium salt *10 mM aqueous solution*