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What are LAMP primers?
Posted June 17, 2022
DNA and RNA QuantitationCell Structures and OrganellesPhysiological ProbesNucleus
Answer
LAMP (loop-mediated isothermal amplification) uses 4 different primers that are specifically designed to recognize 6 distinct regions of the target DNA. The 4 primers used in LAMP include the forward inner primer, forward outer primer, backward inner primer, and backward outer primer. 
  • The forward inner primer (FIP), consists of two regions, one at the 3'end and the other at the 5'end. The two regions are complementary to the F2c region and the F1c region respectively of the template sequence. 
  • The forward outer primer (FOP), has one region that is complementary to the F3c region of the template sequence. 
  • The backward inner primer (BIP) consists of two regions, one at the 3'end and the other at the 5'end. The two regions are complementary to the B2c region and the B1c region respectively of the template sequence. 
  • The backward outer primer (BOP) consists of one region that is complementary to the B3c region of the template sequence.
Additional resources
GLAPD: Whole Genome Based LAMP Primer Design for a Set of Target GenomesLAMP Green™ *50X DMSO Solution*DNA and RNA Quantitation

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