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AAT Bioquest

What are examples of chemiluminescent substrates?

Posted June 24, 2024


Answer

Examples of chemiluminescent substrates used with horseradish peroxidase (HRP) include luminol and acridan-based reagents. Alkaline phosphatase can utilize chemiluminescent substrates such as 4-methylumbelliferyl phosphate (4-MUP) or adamantyl 1,2-dioxetane aryl phosphate (AMPPD). Additionally, luciferase enzymes trigger light emission by oxidizing luciferins. Common chemiluminescent substrates for luciferase include luciferin and coelenterazine.

Additional resources

Chapter Nineteen - Western Blotting using Chemiluminescent Substrates

Luminol [3-Aminophthalhydrazide] *CAS 521-31-3*