Is MTT cell permeable?

Posted October 26, 2022

Answer

Yes, methylthiazolydipheny-tetrazolium bromide (MTT) has strong membrane permeability. MTT produces a yellowish tetrazole solution that is converted to purple, water-insoluble MTT formazan crystals by NADPH-oxidoreductases.

Additional resources

Cell Meter™ Colorimetric MTT Cell Proliferation Kit

Cell Viability Assays

Cell Meter™ Colorimetric WST-8 Cell Quantification Kit

A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity