How do I choose the right wavelength of the spectrophotometer for my experiment?

In absorbance measurements, an IR spectrophotometer utilizes light within the IR range, spanning from 700-15,000 nanometers. On the other hand, a UV-visible instrument operates within the VIS range (400 -700 nanometers) and the UV range (185-400 nanometers). VIS and UV spectroscopy reveal electronic transitions in atoms and molecules. Colorless compounds absorb only in the UV region (usually at a wavelength of 420 nm) while those with color absorb in the visible region. Red light falls between 700-750 nanometers while blue light wavelengths range from 400-450 nanometers, while. Yellow light wavelengths typically fall at 550 nm. Wavelengths below 400 nanometers belong to the UV region and possess higher energy. Overall, optimal wavelengths vary depending on the type of light used, such as visible (VIS), ultraviolet (UV), and infrared (IR).