Why is it important to know the protein concentration accurately?
Posted September 25, 2020
Answer
Accurate protein concentration is paramount to the success of any downstream application that involves protein extraction or analysis. It enables users to compare results between experiments or between proteins with greater precision. It can be used to determine protein yield, measure enzyme kinetics, and during protein extraction it can confirm the success of cell lysis.
Additional resources
The Purification of Proteins Is an Essential First Step in Understanding Their Function.
Amplite™ Fluorimetric Protein Quantitation Kit *Orange Fluorescence*