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AAT Bioquest

What proteins are involved in the DNA replication?

Posted July 22, 2020


Answer

Many proteins and enzymes are involved in the DNA replication, such as:


  • Topoisomerase: It relaxes the DNA from its super-coiled nature.
  • DNA helicase: Helicase separates the two strands of DNA at the replication fork.
  • Single-strand DNA-binding protein: These proteins bind to the ssDNA unwound by helicase and prevent the re-forming of DNA double helix, thus maintaining the strand separation.
  • DNA polymerase: It is responsible for catalyzing the addition of nucleotides to the growing DNA. Polymerases also perform proof-reading and error correction.
  • Primase: It provides a starting point of RNA for DNA polymerase to begin synthesizing the new DNA strand.
  • DNA ligase: They are responsible to join the Okazaki fragments of the lagging strand, forming a continuous strand.

Additional resources

Helixyte™ Green *20X Aqueous PCR Solution*

6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*

Perdew, G. H., Heuvel, J. P. V., & Peters, J. M. (2008). Regulation of gene expression. Springer Science & Business Media.

Lodish, H., Berk, A., Kaiser, C. A., Krieger, M., Scott, M. P., Bretscher, A., ... & Matsudaira, P. (2008). Molecular cell biology. Macmillan.