The type of media used in tissue culturing largely depends on the sample type and should be optimized accordingly. For plant tissue cultures the media generally contains a mixture of macronutrients, micronutrients, vitamins, hormones, nitrogen supplements, amino acids, growth regulators, organic supplements and solidifying agents. For mammalian cells the media contains a mixture of amino acids, vitamins, inorganic salts, glucose and other supplements. 

• Common types of plant tissue culture media include Murashige and Skoog (MS) medium, Linsmaier and Skoog (LS) medium, Gamborg (B5) medium, Nitsch and Nitsch (NN) medium and White’s medium. 
• Common types of mammalian cell culture media include Eagle minimal essential medium (MEM), Dulbecco’s modified Eagle medium (DMEM), RPMI 1640 and HamF10.