What is the working mechanism of Ionomycin?

Posted June 26, 2019

calcium indicator

calcium mobility

Answer

Ionomycin is a calcium ionophore used to increase intracellular calcium concentration, it does so by:

Facilitating the transport of Ca²⁺ across the plasma membrane.
Releasing Ca²⁺ from its intracellular stores without the involvement of G proteins, resulting in the activation of calcium-activated chlorine channels.
Acts on the same intracellular Ca²⁺ stores as 'P3

Additional resources

Mechanism of action of calcium ionophores on intact cells: ionophore-resistant cells

MECHANISM OF RELEASE OF Ca2+ FROM INTRACELLULAR STORES IN RESPONSE TO IONOMYCIN IN OOCYTES OF THE FROG XENOPUS LAEVIS

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