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What is the function of electrophoresis buffer?
Posted June 1, 2020
Answer
Buffers in gel electrophoresis are used to
- Provide ions that are necessary for the passage of electricity
- Maintain the pH at a relatively constant value to make the structure and charge of a protein or nucleic acid stable
- Chelate metal ions to prevent DNA from degradation by DNases in the case of DNA gel electrophoresis where EDTA is added in the running buffer
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