What is the difference between western blotting and far-western blotting?

While western blotting is used to detect proteins, far-western blotting is used to detect protein-protein interactions. This is done by using antibodies for protein detection in western blotting, while using a protein probe in far-western blotting. In western blotting, gel electrophoresis is usually performed under denaturing conditions using SDS, while in far-western blotting, native PAGE (without the use of SDS) may be more beneficial, since it can help to ensure proper protein interactions by maintaining native confirmations.