What is the difference between a PCR primer and a sequencing primer?
Posted February 4, 2020
Answer
The differences between a PCR primer and sequencing primer are outlined in Table 1.
Table 1. Differences between PCR primer and sequencing primer.
Feature | PCR primer | Sequencing primer |
Purpose | DNA amplification | DNA sequencing |
Variations | Depends on the target sequence of the DNA | Specific to the DNA library and the sequencing instrument |
Number | Designed as a pair (forward and reverse primer) | Single |