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AAT Bioquest

What is the difference between a PCR primer and a sequencing primer?

Posted February 4, 2020


Answer

The differences between a PCR primer and sequencing primer are outlined in Table 1.

 

Table 1. Differences between PCR primer and sequencing primer.
FeaturePCR primerSequencing primer
PurposeDNA amplificationDNA sequencing
VariationsDepends on the target sequence of the DNASpecific to the DNA library and the sequencing instrument
NumberDesigned as a pair (forward and reverse primer)Single