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What is the best method for permeabilizing cells?

Posted May 9, 2020


Antibodies and Proteomics
Chemical Reagents
Buffer Preparations and Recipes
Cellular Structures and Organelles
PBS
Permeabilization
buffer
cell lysis
detergent
Answer

Good options for permeabilizing cells are 0.1-0.2% saponin, Tween-20, or TritonX-100 in PBS.

Additional resources

ReadiUse™ 10% Triton X-100 *Hydrogen Peroxide-and Carbonyl-Free*

Tween® 20 Detergent

PBS (Phosphate Buffered Saline) (1X, pH 7.4) Preparation and Recipe

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