What is linearity and dynamic range?

Linearity

Fluorescence intensity is theoretically proportional (linear) to concentration. There are, however, factors that affect this linear relationship. When concentration is too high, light cannot pass through the sample to cause excitation. Thus very high concentrations can have very low fluorescence intensity (concentration quenching). The linearity of a sample is related to many factors, including the chemical composition of the sample and the path length the light must travel. An unknown sample should always be tested for linearity

Dynamic Range

Dynamic range refers to the range of concentrations an instrument can read, from the minimum to the maximum detectable. The minimum detectable concentration is determined by signal-to-noise and signal-to-blank ratios. The maximum detectable concentration is determined by the compound's chemistry and by factors such as instrument sensitivity ranges, optical path length, specificity of optical filters, etc.