What is a typical NAD to NADH ratio?

Posted August 20, 2018

nicotinamide adenine dinucleotide

nicotinamide adenine dinucleotide phosphate

Answer

In mammalian cells, the total NAD/NADH ratio is often cited as 10:1 while free cytoplasmic NAD/NADH ratio is estimated to be roughly 725:1.

Additional resources

Nicotinamide adenine dinucleotide, a metabolic regulator of transcription, longevity and disease.

The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver

Related questions

My NAD+ assay is giving inconsistent results when repeated-do you have any suggestions as to why?

Can Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* be used to quantify NAD and NADH separately? Can this assay measure only NAD? Can this assay measure only NADH?

Is Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* pH sensitive? Do I have to prepare my samples in a certain pH buffer? Will pH influence my fluorescence readings?

Why is my NADH assay showing unreasonably high absorbance readings?

Will Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* work with NADP/NADPH? Can this kit measure NADP+ and NADPH?